AP Lab Information...





Below is all of the information you will need to prepare for the last exam of the year...

You are responsible for printing out the individual labs and bringing them to class on the appropriate day:

The New AP Biology Lab Manual [CLICK HERE]

Official AP Biology Quantitative Skills Guide [CLICK HERE]

These are amazing videos to help you with the labs, stats and general bio [Bozeman Science]:

ALL Anderson Videos Specific to Stats and Graphing[CLICK HERE]


standard deviation, standard error, and graphing [CLICK HERE]
AP Biology Formula Sheet [CLICK HERE]
Review Question Packet [CLICK HERE]
Hardy-Weinberg Problems [CLICK HERE]
Hardy-Weinberg Answers [CLICK HERE]
Chi2 Practice Questions [CLICK HERE]
Chi2 Practice Questions 2 [CLICK HERE]
Water Potential Practice Questions [CLICK HERE]
Water Potential Practice Questions Answers [CLICK HERE]
Population Growth Practice Questions [CLICK HERE]
Population Growth Practice Questions 2 [CLICK HERE]


Q10 - Temp Coefficient - Video (Because I have no idea where else to put it...)




Artificial Selection (Big Idea 1, Lab 1)

For this lab, we will be in the library. It requires Excel. You are supposed to grow plants, but I will give you hypothetical data instead. The characteristic I will use and select upon is plant height. Read the lab as if you were going to do the entire procedure and be prepared to take the lab quiz.

The main goals of this lab other than to understand artificial selection is to learn how to...
1. Calculate standard deviation (see video on standard deviation below)
2. Calculate standard error (see video on standard error below)
3. Use error bars (see video on standard error below)

Hypothetical Data:
1. Original Population
2. F1 Generation

Artificial Selection PowerPoint - [download]


Exponential Growth
http://www.youtube.com/watch?v=c6pcRR5Uy6w


Logistic Growth
http://www.youtube.com/watch?v=rXlyYFXyfIM



Hardy-Weinberg Lab (Big Idea 1, Investigation 2)

In addition to generating the mathematical model you will also need to:
Part I
1. You should have a minimum of two generations in your model
2. Have the model automatically calculate Chi2 values comparing the observed offspring with the expected offspring
Part II
3. Make a second mathematical model showing the outcomes of a classic dihybrid independent assortment between two double heterozygous individuals and have the model also calculate a chi2 between observed phenotypes and expected phenotypes.


Hardy-Weinberg Video
http://www.youtube.com/watch?v=xPkOAnK20kw




BLAST Lab (Big Idea 1, Investigation 3)

[Click Here] for the 2014-15 Lab (UvrC)
[Blast]

BLAST video
http://www.youtube.com/watch?v=OSKwuOccAak



Cladogram Video
http://www.youtube.com/watch?v=ouZ9zEkxGWg&feature=youtu.be


Osmosis and Diffusion (Big Idea 2, Lab 4)

**This lab is almost identical to Lab 1 of the old AP Curriculum.
Therefore, we will use the old procedure...download [this] and prepare - we are only doing exercise 1C/1D in class - determining water potential of potato cells...

PowerPoint that goes with the lab [click here]



What you will do:
1. Collect group data (percent change in mass)
2. Post the data [here] for all groups to share
3. On an EXCEL spreadsheet calculate mean, SD, SE for each sucrose concentration and then graph (its a "linear" relationship and therefore you use a line of best fit...not connect the dots - dont forget error bars).
4. Based on graph determine the isotonic condition and then the water potential.
5. Determine the concentration of the unknown solution and post answer on spreadsheet with explanation of how you determined this.
6. Do the questions from 1C/1D (pages 14 through 16) directly on the spreadsheet...turn in spreadsheet via edmodo to ME.

Osmosis Video
http://www.youtube.com/watch?v=LeS2-6zHn6M



Photosynthesis (Big Idea 2, Lab 5)

PowerPoint: [click here]

Photosynthesis Experiment Video
http://www.youtube.com/watch?v=ZnY9_wMZZWI


Cellular Respiration (Big Idea 2, Lab 6)

**This lab is almost identical to Lab 5 of the old AP Curriculum.
Therefore, we will use the old procedure...download [this] and study for lab quiz.

Here is a Bozeman video to help you understand what you will be doing:



What you need to turn in...
1. Follow the instructions in this PowerPoint [click here]
2. Make sure to design a new experiment where it says "Designing and Conducting your own Experiment". You need not answer the series of questions below this heading on paper. They are just to give you ideas. Just design an experiment.


Mitosis and Meiosis (Big Idea 3, Lab 7)


1. PowerPoint Description [click here]
2. Bozeman video - focues on ascomycetes/meiosis part:
http://www.youtube.com/watch?v=pOROHmEmqmU




Biotechnology - Transformation, Restriction Digest, and Electrophoresis (Big Idea 3, Labs 8 and 9)

1. PowerPoint Description for Lab 8 - Transformation [click here]
Make sure you understand how to calculate transformation efficiency
2. PowerPoint Description for Lab 9 - Electrophoresis, DNA fingerprinting, RFLPs [click here]
3. This video should help you recall these techniques http://www.youtube.com/watch?v=yYIZgS-L5Sc



Transpiration (Big Idea 4, Lab 11)

Transpiration Lab video
http://www.youtube.com/watch?v=FEfmTok3OCo



Water Potential Video
http://www.youtube.com/watch?v=nDZud2g1RVY


Enzyme Activity (Big Idea 4, Lab 13)


1. PowerPoint Description of Actual Lab [click here]
2. Enzyme Lab video - old AP Enzyme Lab, but still useful
http://www.youtube.com/watch?v=hs75JmgGW5Y






Additional Labs you should be aware of:


Animal Behavior


Lab video - pill bug lab with chi square
http://www.youtube.com/watch?v=jSKkecFzD50



Descrcriptive Stats and Graphing You Should Understand:


Click here for ALL Anderson Videos Specific to Stats and Graphing

Chi-Squared Test
(http://www.youtube.com/watch?v=WXPBoFDqNVk)::


Standard deviation video
(http://www.youtube.com/watch?v=09kiX3p5Vek)::


Standard error / error bars video
(http://www.youtube.com/watch?v=BwYj69LAQOI):



FINAL REVIEW

Contains problems including chi2, hardy-weinberg, and waterpotential:

http://www.youtube.com/watch?v=BpaqvCrNAgI



Old School AP Bio Labs




Lab 7: Genetics

Before coming to class you are being asked to:

1. Complete the "statistical analysis section" of the lab
2. To watch "Mr. Anderson's" presentation on youtube found [here] and below
3. To visit the fly lab and familiarize yourself with the [website]
4. To print out the actual lab 7 and the one being used in class below:
To download the lab with the stats section [Click here].
To download the actual lab being used in class [Click here].


The bozeman video that goes with the lab:

Lab 8: Primer Design

Primer Design Lab [CLICK HERE]




How will labs be graded?

1. 10% from the pre-lab quiz
A quiz will be given before the lab based on the lab's content, purpose and procedure (Everything you should know before doing the lab basically). The question that is typically asked is "What are you doing today and why?"

2. 30% from your in-class lab technique and performance
You will be observed during the lab in terms of proper lab behaivor, technique and safety. It will quickly be evident if you understand the lab or not, which is why you should know the lab really well before coming in to do it.

3. 60% from your lab write-up, some of which will be a formal manuscript (see below).


How do I write-up my lab

manuscript?


1. All manuscript will be formatted according to the European Molecular Biology Organization (EMBO) Jounral analogous to what research scientists do with their observations.

2. Read the "guide for authors" as to how EMBO wants the manuscript formatted.

3. You can [Click Here] for an actual manuscript that was submitted to EMBO. This is what your manuscript should look like.

4. [CLICK HERE] for a guide on the type of information found in each section of a scientific manuscript.

All lab manuscripts must be sent directly to me using edmodo by the due date.


These are the old curriculum labs, but many are still the same:

Need a reminder?

1.[CLICK HERE] to view an online tutorial of all 12 AP labs

2.[CLICK HERE] AP Biology Weblabs - simulation of all the AP labs

3.[CLICK HERE] For yet another AP Biology lab resource